Co-Immobilization and Compartmentalization of Cholesterol Oxidase, Glucose Oxidase and Horseradish Peroxidase for Improved Thermal and H2o2 Stability
نویسندگان
چکیده
منابع مشابه
Covalent attachment of cholesterol oxidase and horseradish peroxidase on perlite through silanization: activity, stability and co-immobilization.
In the present work, co-immobilization of cholesterol oxidase (COD) and horseradish peroxidase (POD) on perlite surface was attempted. The surface of perlite were activated by 3-aminopropyltriethoxysilane and covalently bonded with COD and POD via glutaraldehyde. Enzymes activities have been assayed by spectrophotometric technique. The stabilities of immobilized COD and POD to pH were higher th...
متن کاملEnzymatic growth of quantum dots: applications to probe glucose oxidase and horseradish peroxidase and sense glucose.
Three innovative assays are developed for the detection of enzymatic activities of glucose oxidase (GOx) and horseradish peroxidase (HRP) by the generation of CdS quantum dots (QDs) in situ using non-conventional enzymatic reactions. In the first assay, GOx catalyzes the oxidation of 1-thio-β-D-glucose to give 1-thio-β-D-gluconic acid. The latter is spontaneously hydrolyzed to β-D-gluconic acid...
متن کاملDirect comparison of gluco-oligosaccharide oxidase variants and glucose oxidase: substrate range and H2O2 stability
Glucose oxidase (GO) activity is generally restricted to glucose and is susceptible to inactivation by H2O2. By comparison, the Y300A variant of gluco-oligosaccharide oxidase (GOOX) from Sarocladium strictum showed broader substrate range and higher H2O2 stability. Specifically, Y300A exhibited up to 40 times higher activity on all tested sugars except glucose, compared to GO. Moreover, fusion ...
متن کاملEnhancement of Functional Properties of Cholesterol Oxidase and Glucose Oxidase enzymes through Co-Immobilization on Organic Support
Glucose and cholesterol are biomolecules of prime importance to human life as they are associated with a number of diseases such as diabetes, cardiac diseases, kidney failure, etc. Quantitative determination of these biomolecules is very important in biochemistry, clinical chemistry and food biology. Numerous methods such as spectrometry, amperometry, HPLC, polarometry, and capillary electropho...
متن کاملExtracellular Cholesterol Oxidase from Rhodococcus sp.: Isolation and Molecular Characterization
Background: Cholesterol oxidase (CHO) has various clinical and industrial applications. Recently, microbial CHO have received a great attention for their wide usage in medicine. Here, taxonomic characterizations of isolated strain from soil, optimization of the conditions for CHO production and biochemical characterizations of produced CHO enzyme were described. Finally, CHO gene was cloned int...
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ژورنال
عنوان ژورنال: Social Science Research Network
سال: 2022
ISSN: ['1556-5068']
DOI: https://doi.org/10.2139/ssrn.4134284